How do you calculate peptide reconstitution?

Divide the peptide mass in the vial (mg) by the volume of bacteriostatic water added (mL) to get the concentration in mg/mL. To find the volume for a target dose, divide the dose by the concentration. On a U-100 insulin syringe, 1 mL equals 100 units.

What are insulin syringe units for a peptide dose?

A U-100 insulin syringe is marked so that 100 units equal 1 milliliter. Multiply the draw volume in milliliters by 100 to read the dose directly on the syringe in units.

Tools

Peptide reconstitution calculator

Enter the peptide mass in the vial, the volume of bacteriostatic water you plan to add, and your target research dose. Get the concentration, the volume to draw, the reading on a U-100 insulin syringe, and how many doses the vial yields.

Peptide in vial (mg)Bacteriostatic water added (mL)Target dose (mcg)

Draw volume per dose: 0.050 mL; 5.0 units on a U-100 insulin syringe
Concentration: 5.00 mg/mL; 5,000 mcg/mL
Doses per vial: 40.0

U-100 syringe: 100 units = 1 mL. Figures are arithmetic conversions for laboratory handling and reference only.

For Research Use Only

This material is supplied strictly for in-vitro laboratory and preclinical research. Not for human consumption, veterinary use, diagnostic, or therapeutic purposes.

How the math works

Reconstitution concentration is simply the peptide mass divided by the diluent volume: a 10 mg vial reconstituted with 2 mL of bacteriostatic water gives 5 mg/mL, or 5,000 mcg/mL. To pull a 250 mcg research aliquot from that vial you divide 250 by 5,000, which is 0.05 mL — read as 5 units on a U-100 insulin syringe.

Adding more water lowers the concentration and increases the draw volume for the same dose, which can make small aliquots easier to measure accurately. The peptide quantity itself never changes — only the volume it is dissolved in.

For research use only

This calculator is provided for in-vitro laboratory and preclinical reference. Nothing here is dosing guidance for humans or animals.